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ScienCellGK103人前脂肪細胞生物學(xué)qPCR檢測試劑盒的特點
更新時間:2023-01-10   點擊次數(shù):544次

GK103 ScienCell 人前脂肪細胞生物學(xué)qPCR檢測試劑盒,Human Preadipocyte Cell Biology qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務(wù)標準。

GK103 ScienCell人前脂肪細胞生物學(xué)qPCR檢測試劑盒,Human Preadipocyte Cell Biology qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK103

產(chǎn)品名稱:GK103 ScienCell人前脂肪細胞生物學(xué)qPCR檢測試劑盒,Human Preadipocyte Cell Biology qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

北京裕恒豐科技有限公司是ScienCell中國區(qū)總代理,提供各種基因表達分析相關(guān)品,ScienCell細胞培養(yǎng)基、轉(zhuǎn)染試劑、細胞檢測試劑盒等細胞生物學(xué)產(chǎn)品。

GK103 ScienCell人前脂肪細胞生物學(xué)qPCR檢測試劑盒,Human Preadipocyte Cell Biology qPCR Array Kit, 產(chǎn)品說明:

前脂肪細胞是成纖維細胞樣前體細胞,可以增殖并分化成成熟的脂肪細胞。前脂肪細胞在代謝穩(wěn)態(tài)和營養(yǎng)信號傳導(dǎo)中起重要作用。ScienCell GeneQuery™ 人類前脂肪細胞生物學(xué) qPCR 陣列試劑盒 (GQH-PAD) 旨在促進參與人類前脂肪細胞生物學(xué)的 88 個關(guān)鍵基因的基因表達譜分析,例如細胞增殖、分化、ECM 形成和脂質(zhì)代謝。與肥胖和脂肪肉瘤有關(guān)的基因也包含在陣列中。下面顯示了如何根據(jù)其功能對基因進行分組的簡要示例:  

前脂肪細胞標志物:ADIPOQLEP,HOXC8 / 9,TBX1TMEM26,UCP1CIDEA,PRDM16,SLC7A10P2RX5

增殖: CCND1, PROKR1, LYRM1, FTO, SLC2A4, PDGFA, IL4, IGF1, FGF5 MAPK1/3, TNFSF10

分化和脂肪生成:PPARG,CEBPA/BKLF2/5/15,SOCS1/3INSR,DLK1,ADD1,SREBF1

ECM 和細胞骨架: MMP1/2/3, FAP, DPP4 TGFB1, COL1A1/2 SMAD2/4

脂質(zhì)代謝: FABP5/7, LBP LPL, PLIN2, ACSL1, LIPC

肥胖癥: MC4R, UCP3, ADRB2/3 POMC, FFAR4, CARTPT

脂肪肉瘤: FUS, MDM2 HMGA2, DDIT3, CDK4 EWSR1, DES, VIM

注意:所有基因名稱都遵循人類基因組組織基因命名委員會(HGNC)的符號。

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

Preadipocytes are fibroblast-like precursor cells that can proliferate and differentiate into mature adipocytes. Preadipocytes play an important role in metabolic homeostasis and nutrient signaling. ScienCell's GeneQuery™ Human Preadipocyte Cell Biology qPCR Array Kit (GQH-PAD) is designed to facilitate gene expression profiling of 88 key genes involved in human preadipocyte cell biology, such as cell proliferation, differentiation, ECM formation and lipid metabolism. Genes implicated in obesity and liposarcoma are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:    

·         Preadipocyte cell markers: ADIPOQ, LEP, HOXC8/9, TBX1, TMEM26, UCP1, CIDEA, PRDM16, SLC7A10, P2RX5

·         Proliferation: CCND1, PROKR1, LYRM1, FTO, SLC2A4, PDGFA, IL4, IGF1, FGF5, MAPK1/3, TNFSF10

·         Differentiation and adipogenesis: PPARG, CEBPA/B, KLF2/5/15, SOCS1/3, INSR, DLK1, ADD1, SREBF1

·         ECM and cytoskeleton: MMP1/2/3, FAP, DPP4, TGFB1, COL1A1/2, SMAD2/4

·         Lipid metabolism: FABP5/7, LBP, LPL, PLIN2, ACSL1, LIPC

·         Obesity: MC4R, UCP3, ADRB2/3, POMC, FFAR4, CARTPT

·         Liposarcoma: FUS, MDM2, HMGA2, DDIT3, CDK4, EWSR1, DES, VIM

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人前脂肪細胞細胞生物學(xué)qPCR陣列板布局 *8 個粗體和斜體控件)

注意:所有基因名稱都遵循HGNC的符號


1

2

3

4

5

6

7

8

9

10

11

12

A

ACSL1

CDK4

DES

FAP

HMGA2

KLF15

LTBP1

MMP3

PRDM16

SLC7A10

TMEM26

ACTB

B

ADD1

CEBPA

DGAT2

FASLG

HOXC8

KLF2

LYRM1

P2RX5

PROKR1

SMAD2

TNF

GAPDH

C

ADIPOQ

CEBPB

DLK1

FFAR4

HOXC9

KLF5

MAPK1

PDGFA

PTGR1

SMAD4

TNFSF10

LDHA

D

ADRB2

CIDEA

DPP4

FGF5

IGF1

LBP

MAPK3

PDGFRA

RUNX2

SOCS1

UCP1

NONO

E

ADRB3

COL1A1

EWSR1

FTO

IL4

LEP

MC4R

PLIN2

SDC3

SOCS3

UCP3

PPIH

F

AGRP

COL1A2

FABP4

FUS

INPPL1

LIPC

MDM2

PNPLA2

SIM1

SREBF1

VIM

GDC

G

CARTPT

COL6A3

FABP5

GATA3

INSR

LIPE

MMP1

POMC

SLC2A4

TBX1

WISP2

PPC

H

CCND1

DDIT3

FABP7

GLI3

KITLG

LPL

MMP2

PPARG

SLC36A2

TGFB1

WNT5A

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息:

GK001   人類細胞凋亡qPCR檢測試劑盒,Human Apoptosis qPCR Array Kit

GK002   人類多能干細胞qPCR檢測試劑盒,Human Pluripotent Stem Cell qPCR Array Kit

GK003   人類細胞周期qPCR檢測試劑盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮細胞生物學(xué)qPCR檢測試劑盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運qPCR檢測試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人類星型膠質(zhì)細胞生物學(xué)qPCR檢測試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細胞發(fā)育和色素沉著qPCR檢測試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細胞生物學(xué)qPCR檢測試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質(zhì)細胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內(nèi)皮細胞表型異質(zhì)性qPCR檢測試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內(nèi)皮細胞生物學(xué)qPCR檢測試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內(nèi)細胞分化qPCR檢測試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應(yīng)力和機械傳導(dǎo)qPCR檢測試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號通路qPCR檢測試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應(yīng)qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號響應(yīng)qPCR檢測試劑盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬細胞細胞生物學(xué)qPCR檢測試劑盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人類吞噬作用qPCR檢測試劑盒,Human Phagocytosis qPCR Array Kit

GK039   人類先天免疫反應(yīng)qPCR檢測試劑盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纖維細胞生長因子信號通路qPCR檢測試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測試劑盒,Human Skin Wound Healing qPCR Array Kit

GK056   人體止血qPCR檢測試劑盒,Human Hemostasis qPCR Array Kit

GK060   人類晝夜節(jié)律qPCR檢測試劑盒,Human Circadian Rhythm qPCR Array Kit

GK061   人類DNA損傷感應(yīng)qPCR檢測試劑盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人類細胞生長和分裂qPCR檢測試劑盒,Human Cell Growth and Division qPCR Array Kit

GK063   人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人類小膠質(zhì)細胞生物學(xué)qPCR檢測試劑盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人類適應(yīng)性免疫反應(yīng)qPCR檢測試劑盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

GK074   人角質(zhì)細胞生物學(xué)qPCR檢測試劑盒,Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細胞生物學(xué)qPCR檢測試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細胞生物學(xué)qPCR檢測試劑盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人類周細胞生物學(xué)qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

GK084   人體軸突導(dǎo)向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

GK087   人類染色質(zhì)組織和重塑qPCR檢測試劑盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088   VEGF信號通路qPCR檢測試劑盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人類Ephrin信號qPCR檢測試劑盒,Human Ephrin Signaling qPCR Array Kit

GK090   人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit

GK091   人肝星狀細胞生物學(xué)qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星狀細胞生物學(xué)qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人類雪旺細胞生物學(xué)qPCR檢測試劑盒,Human Schwann Cell Biology qPCR Array Kit

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GK100   人角質(zhì)形成細胞生物學(xué)qPCR檢測試劑盒,Human Keratinocyte Cell Biology qPCR Array Kit

GK101   人類Synoviocyte細胞生物學(xué)qPCR檢測試劑盒,Human Synoviocyte Cell Biology qPCR Array Kit

GK102   人類滋養(yǎng)層細胞生物學(xué)qPCR檢測試劑盒,Human Trophoblast Cell Biology qPCR Array Kit

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GK104   人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit

GK105   人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit

GK106   人類焦點粘附復(fù)合物qPCR檢測試劑盒,Human Focal Adhesion Complexes qPCR Array Kit

GK107   人內(nèi)皮素信號qPCR檢測試劑盒,Human Endothelin Signaling qPCR Array Kit

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GK113   人類神經(jīng)干細胞標記qPCR檢測試劑盒,Human Neural Stem Cell Markers qPCR Array Kit

GK114   人類小膠質(zhì)細胞極化標記qPCR檢測試劑盒,Human Microglial Polarization Markers qPCR Array Kit

GK115   人類骨髓譜系標記qPCR檢測試劑盒,Human Myeloid Lineage Markers qPCR Array Kit

GK116   B細胞和T細胞發(fā)育標記qPCR檢測試劑盒,Human B and T Cells Development Markers qPCR Array Kit

GK117   人胸腺細胞發(fā)育標記qPCR檢測試劑盒,Human Thymocyte Development Markers qPCR Array Kit

GK118   人類先天淋巴細胞發(fā)育標記qPCR檢測試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119   人類神經(jīng)祖細胞標記qPCR檢測試劑盒,Human Neural Progenitor Markers qPCR Array Kit

GK121   PD-1/PD-L1檢查點途徑qPCR檢測試劑盒,GeneQuery Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit

GK123   人類生長因子qPCR檢測試劑盒,Human Growth Factors qPCR Array Kit

GK124   人類轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Transcription Factors qPCR Array Kit

GK125   人干細胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細胞表面標記qPCR檢測試劑盒,Human Cell Surface Markers qPCR Array Kit

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