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ScienCellGK084人體軸突導向qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):532次

GK084 ScienCell 人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK084 ScienCell人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit,產品簡介:

產品品牌: ScienCell

產品貨號:GK084

產品名稱:GK084 ScienCell人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

產品規(guī)格:One 96-well plate, one 20ul reaction per well


GK084 ScienCell人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit 產品說明:

ScienCellGeneQuery™人類軸突引導qPCR陣列(GQH-AXN)分析了88個參與神經尋路的關鍵基因。 在神經發(fā)育過程中,神經元發(fā)送投影(也稱為軸突)以到達目標并傳輸電信號。 軸突遵循特定的路徑,潛在的尋路機制是一個不斷增長的研究領域。 以下是如何根據(jù)其信號通路或蛋白質類別對所包含基因進行分組的簡要示例:

腎上腺素: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1

信號素: SEMA3A, SEMA4D, SEMA5A SEMA6B, SEMA7A

WNT 信令: WNT4, WNT5A, SHH, SMO FZD3

Netrin信令:NTN1,NTN4,NTNG1,NTNG2,DCC,UNC5A

細胞粘附分子:CAMK2A、CAMK2B、CAMK2D、CAMK2G

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Axon Guidance qPCR Array (GQH-AXN) profiles 88 key genes involved in neural pathfinding.  During neural development, neurons send projections otherwise known as axons to reach targets and transmit electric signals.  Axons follow specific paths and the underlying pathfinding mechanism is an area of growing research.  Below are brief examples of how included genes may be grouped according to their signaling pathways or protein classes:

·         Ephrins: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1

·         Semaphorins: SEMA3A, SEMA4D, SEMA5A, SEMA6B, SEMA7A

·         WNT signaling: WNT4, WNT5A, SHH, SMO, FZD3

·         Netrin signaling: NTN1, NTN4, NTNG1, NTNG2, DCC, UNC5A

·         Cell adhesion molecules: CAMK2A, CAMK2B, CAMK2D, CAMK2G

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類軸突引導 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ABL1

CAMK2B

EFNA1

EPHA1

EPHB3

NRP1

RAC1

SEMA3D

SEMA4F

SLIT2

TRPC6

β-actin

B

ABLIM3

CAMK2D

EFNA2

EPHA2

EPHB4

NRP2

RAF1

SEMA3E

SEMA4G

SLIT3

UNC5A

GAPDH

C

ARHGEF12

CAMK2G

EFNA3

EPHA3

F2RL2

NTN1

ROBO1

SEMA3F

SEMA5A

SMO

UNC5B

LDHA

D

BMP7

CFL1

EFNA4

EPHA4

FES

NTN4

ROBO2

SEMA3G

SEMA6B

TRPC1

UNC5C

NONO

E

BMPR1B

CFL2

EFNA5

EPHA5

FYN

NTNG1

ROCK1

SEMA4A

SEMA6D

TRPC1

UNC5D

PPIH

F

BMPR2

CXCR4

EFNB1

EPHA7

FZD3

NTNG2

RYK

SEMA4B

SEMA7A

TRPC3

WNT4

GDC

G

BOC

DCC

EFNB2

EPHB1

NCK1

PAK2

SEMA3A

SEMA4C

SHH

TRPC4

WNT5A

PPC

H

CAMK2A

DPYSL2

EFNB3

EPHB2

NFATC2

PAK4

SEMA3C

SEMA4D

SLIT1

TRPC5

WNT5B

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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