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ScienCellGK034人肺纖維化qPCR檢測(cè)試劑盒
更新時(shí)間:2023-01-03   點(diǎn)擊次數(shù):383次

GK034 ScienCell 人肺纖維化qPCR檢測(cè)試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來(lái)源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開(kāi)發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK034 ScienCell 人肺纖維化qPCR檢測(cè)試劑盒,Human Pulmonary Fibrosis qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號(hào):GK034

產(chǎn)品名稱:GK034 ScienCell 人肺纖維化qPCR檢測(cè)試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK034 ScienCell 人肺纖維化qPCR檢測(cè)試劑盒,Human Pulmonary Fibrosis qPCR Array Kit, 產(chǎn)品說(shuō)明:

ScienCellGeneQuery™人肺纖維化qPCR陣列試劑盒(GQH-PFS)旨在促進(jìn)88個(gè)參與肺纖維化的關(guān)鍵基因的基因表達(dá)譜分析。肺纖維化是由傷口愈合和組織重塑過(guò)程中細(xì)胞外基質(zhì)(ECM)沉積過(guò)多引起的。隨著時(shí)間的推移,肺部瘢痕形成會(huì)導(dǎo)致組織增厚、僵硬和呼吸問(wèn)題。如何根據(jù)其功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:  

肺表面活性蛋白:SFTPA2,SFTPAC

炎癥趨化因子:CCL2CCL3,CCL11,CCR2CCR3,CXCL8

炎性細(xì)胞因子:PDGFA,CSF2IL5,IL6,IL13

ECM 重塑: LOX, MMP1, MMP2 MMP8, PLAT, PLAU, PLG SERPINA1, SERPINE2, TIMP1, TIMP2, TIMP3

生長(zhǎng)因子:EGFIGF1,HGF,FGF1,FGF2,AGTEDN1,CTGF

TGFB信號(hào)通路:SKIL,SPP1,TGFB1TGFB2

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可以特異性識(shí)別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說(shuō)明,否則引物組可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過(guò)qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Pulmonary Fibrosis qPCR Array Kit (GQH-PFS) is designed to facilitate gene expression profiling of 88 key genes involved in pulmonary fibrosis. Pulmonary fibrosis is caused by excessive extracellular matrix (ECM) deposition during wound healing and tissue remodeling. The scarring of lung leads to thickened, stiff tissues and respiratory problems over time. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Pulmonary surfactant proteins: SFTPA2, SFTPAC

·         Inflammatory chemokines: CCL2, CCL3, CCL11, CCR2, CCR3, CXCL8

·         Inflammatory cytokines: PDGFA, CSF2, IL5, IL6, IL13

·         ECM remodeling: LOX, MMP1, MMP2, MMP8, PLAT, PLAU, PLG, SERPINA1, SERPINE2, TIMP1, TIMP2, TIMP3

·         Growth factors: EGF, IGF1, HGF, FGF1, FGF2, AGT, EDN1, CTGF

·         TGFB signaling pathway: SKIL, SPP1, TGFB1, TGFB2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人肺纖維化qPCR陣列板布局*8 個(gè)粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CCL3

CXCL8

FAM13A

HPS3

IL13

MMP1

NFE2L2

PLAU

SFTPD

TGFA

β-actin

B

AGT

CCL5

CYSLTR2

FGF1

HPS4

IL13RA2

MMP13

NFKB1

PLG

SKIL

TGFB1

GAPDH

C

ATP11A

CCR2

DSP

FGF2

HPS5

IL1B

MMP2

NFKB2

PTX3

SMAD3

TGFB2

LDHA

D

BMP7

CCR3

EDN1

FGF7

HPS6

IL5

MMP3

OBFC1

SERPINA1

SMAD7

TIMP1

NONO

E

CALCA

CEBPB

EGF

GREM1

IFNG

IL6

MMP8

PARN

SERPINE1

SNAI1

TIMP2

PPIH

F

CCL11

CSF2

ELMOD2

HGF

IGF1

LOX

MMP9

PDGFA

SERPINH1

SPP1

TIMP3

GDC

G

CCL18

CSF3

ELN

HMOX1

IL10

LPAR1

MT2A

PDGFB

SFTPA2

TERC

TIMP4

PPC

H

CCL2

CTGF

FAM111B

HPS1

IL12B

MECP2

MUC5B

PLAT

SFTPC

TERT

TNF

NTC

提示:基因選擇可能會(huì)根據(jù)新的研究和開(kāi)發(fā)進(jìn)行更新

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GK011   人類黑素細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

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GK025   人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit

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GK123   人類生長(zhǎng)因子qPCR檢測(cè)試劑盒,Human Growth Factors qPCR Array Kit

GK124   人類轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Transcription Factors qPCR Array Kit

GK125   人干細(xì)胞轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細(xì)胞表面標(biāo)記qPCR檢測(cè)試劑盒,Human Cell Surface Markers qPCR Array Kit

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