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ScienCellGK028人類Wnt信號靶基因qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):538次

GK028 ScienCell 人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK028 ScienCell人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK028

產(chǎn)品名稱:GK028 ScienCell人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

GK028 ScienCell人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人類 Wnt 信號靶基因 qPCR 陣列試劑盒 (GQH-WTG) 旨在促進對 Wnt 信號激活有反應的 88 個關鍵基因和 Wnt 信號誘導轉錄下游基因的基因表達譜分析。 該試劑盒包括由規(guī)范和非規(guī)范Wnt信號通路調(diào)節(jié)的基因。 如何根據(jù)其各自的功能對所包含的下游基因進行分組的簡要示例如下所示:

轉錄因子:HNF1A、LEF1TCF7L1、TCF7L2FOSL1

細胞循環(huán)/生長:CCND1,CDKN2A,BIRC5APC,TERC

動力: S100A4 S100A6, NRCAM, KRT1 FN1

血管生成:EDN1,PLAU,VEGFANOTCH1,EDNRA

癌癥失調(diào):TWIST1RET,JUN,NEDD9MLLT10

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Wnt Signaling Target Genes qPCR Array Kit (GQH-WTG) is designed to facilitate gene expression profiling of 88 key genes responsive to Wnt signaling activation and genes downstream of Wnt signaling induced transcription.  This kit includes genes regulated by both canonical and noncanonical Wnt signaling pathways.  Brief examples of how included downstream genes may be grouped according to their respective functions are shown below:

·         Transcription factors: HNF1A, LEF1, TCF7L1, TCF7L2, FOSL1

·         Cell cycling/growth: CCND1, CDKN2A, BIRC5, APC, TERC

·         Motility: S100A4, S100A6, NRCAM, KRT1, FN1

·         Angiogenesis: EDN1, PLAU, VEGFA, NOTCH1, EDNRA

·         Cancer misregulation: TWIST1, RET, JUN, NEDD9, MLLT10

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類 Wnt 信號靶基因 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ABCB1

CCND1

DOT1L

EP300

HES1

L1CAM

MSL1

NOTCH2

PRL

SOX2

TNFRSF19

β-actin

B

AMER1

CD44

EDN1

FGF18

HNF1A

LEF1

MYC

NRCAM

PTGS2

SOX9

TWIST1

GAPDH

C

APC

CDH1

EDN3

FGF20

ID2

MET

MYCN

PEX2

RET

SP5

TWIST2

LDHA

D

ATOH1

CDKN2A

EDNRA

FGF4

IGF1

MLLT10

NANOG

PITX2

RUNX2

TBC1D9

VCAN

NONO

E

BIRC5

CDX1

EGFR

FN1

IL6

MMP14

NANOS2

PLAU

S100A4

TCF7L1

VEGFA

PPIH

F

BMP4

CLDN1

EGR1

FOSL1

IRS1

MMP2

NDP

PLAUR

S100A6

TCF7L2

WISP1

GDC

G

CACNA1G

CTLA4

EGR2

GBX2

JUN

MMP7

NEDD9

POSTN

SOST

TERC

WISP2

PPC

H

CCNA2

CXCL8

EMP1

GJA1

KRT1

MMP9

NOTCH1

PPARD

SOX17

TIAM1

YAP1

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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