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ScienCellGK025人類Notch信號(hào)通路qPCR檢測(cè)試劑盒
更新時(shí)間:2023-01-03   點(diǎn)擊次數(shù):504次

ScienCell GK025 人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來(lái)源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開(kāi)發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK025 ScienCell人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

產(chǎn)品品牌:  ScienCell

產(chǎn)品貨號(hào):GK025

產(chǎn)品名稱:GK025 ScienCell人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

GK025 ScienCell人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit, 產(chǎn)品說(shuō)明:

ScienCell GeneQuery Human Notch 信號(hào)通路 qPCR 芯片試劑盒 (GQH-NCH) 旨在促進(jìn)參與人類 Notch 分子通路的 88 個(gè)關(guān)鍵基因的基因表達(dá)譜分析,重點(diǎn)是 Notch1 信號(hào)轉(zhuǎn)導(dǎo)。 如何根據(jù)其作用對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:

規(guī)范配體:JAG1、JAG2、DLL1DLL3DLL4

非規(guī)范配體:WNT1,DNERCNTN1,NOV,SHH

受體:缺口1,缺口2,缺口3,缺口4

轉(zhuǎn)錄抑制因子:HEY1、HEY2ESR1、HES1、HES7

蛋白水解:ADAM17、PSEN1、FURINPOFUT1、PSENEN

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說(shuō)明,否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過(guò)qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Notch Signaling Pathway qPCR array kit (GQH-NCH) is designed to facilitate gene expression profiling of 88 key genes involved in the human Notch molecular pathway with an emphasis on Notch1 signal transduction.  Brief examples of how included genes may be grouped according to their roles are shown below:

·         Canonical ligands: JAG1, JAG2, DLL1, DLL3DLL4

·         Noncanonical ligands: WNT1, DNER, CNTN1, NOV, SHH

·         Receptors: NOTCH1, NOTCH2, NOTCH3, NOTCH4

·         Transcriptional repressors: HEY1, HEY2, ESR1, HES1, HES7

·         Proteolysis: ADAM17, PSEN1, FURIN, POFUT1, PSENEN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類缺口信號(hào)通路 qPCR 陣列板布局*8 個(gè)粗體和斜體對(duì)照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACBD3

CDKN1A

DLL1

DVL2

GSK3B

IL2RA

MAML3

NCOR2

NRARP

PSENEN

SMAD2

β-actin

B

ADAM10

CIR1

DLL3

EGFL7

HES1

JAG1

MEF2C

NEURL1

NUMB

PTF1A

SMO

GAPDH

C

ADAM17

CLTA

DLL4

ESR1

HES5

JAG2

MFAP2

NFKB2

NUMBL

RBPJ

SNW1

LDHA

D

APH1A

CNTN1

DNAAF3

ESR2

HES7

JUN

MFAP5

NOTCH1

PCSK5

RFNG

SPEN

NONO

E

AXIN1

CNTN6

DNER

FBXW7

HEY1

LFNG

MFNG

NOTCH2

POFUT1

RITA1

TBP

PPIH

F

CCND1

CTNNB1

DNM1

FHL1

HEY2

MAGI2

MIB1

NOTCH3

POGLUT1

RUNX1

TCF7L2

GDC

G

CDK2

DLK1

DTX1

FURIN

HEYL

MAML1

MIB2

NOTCH4

PSEN1

SHH

THBS2

PPC

H

CDK8

DLK2

DTX4

GATA3

HIF1A

MAML2

MLLT4

NOV

PSEN2

SIRT1

WNT1

NTC

提示:基因選擇可能會(huì)根據(jù)新的研究和開(kāi)發(fā)進(jìn)行更新

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